EEF2 Antikörper (AA 1-858)

Produktnummer ABIN7599561

Dieser Anti-EEF2 Antikörper ist ein Kaninchen Polyklonal Antikörper zur Detektion von EEF2 in WB, IHC und ELISA. Geeignet für Human, Maus, Ratte, Zebrafisch (Danio rerio) und Affe.

Kurzübersicht für EEF2 Antikörper (AA 1-858) (ABIN7599561)

Target: EEF2 (Eukaryotic Translation Elongation Factor 2 (EEF2))

Reaktivität: Human, Maus, Ratte, Zebrafisch (Danio rerio), Affe

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser EEF2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Produktdetails anti-EEF2 Antikörper

Bindungsspezifität: AA 1-858

Verwendungszweck: Anti-EEF2 Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Produktmerkmale: Anti-EEF2 Antibody Picoband® (ABIN7599561). Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat, Zebrafish. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human EEF2 recombinant protein (Position: M1-L858).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.25 μg/mL, Human, Monkey, Mouse, Rat, Zebrafish
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse, Rat
ELISA, 0.1-0.5 μg/mL, -
1. Davydova, E., Ho, A. Y. Y., Malecki, J., Moen, A., Enserink, J. M., Jakobsson, M. E., Loenarz, C., Falnes, P. O. Identification and characterization of a novel evolutionarily conserved lysine-specific methyltransferase targeting eukaryotic translation elongation factor 2 (eEF2). J. Biol. Chem. 289: 30499-30510, 2014. 2. Hekman, K. E., Yu, G.-Y., Brown, C. D., Zhu, H., Du, X., Gervin, K., Undlien, D. E., Peterson, A., Stevanin, G., Clark, H. B., Pulst, S. M., Bird, T. D., White, K. P., Gomez, C. M. A conserved eEF2 coding variant in SCA26 leads to loss of translational fidelity and increased susceptibility to proteostatic insult. Hum. Molec. Genet. 21: 5472-5483, 2012. 3. Kaneda, Y., Hayes, H., Uchida, T., Yoshida, M. C., Okada, Y. Regional assignment of five genes on human chromosome 19. Chromosoma 95: 8-12, 1987.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu EEF2

Target: EEF2 (Eukaryotic Translation Elongation Factor 2 (EEF2))

Andere Bezeichnung: EEF2

Hintergrund:

Synonyms: Protein argonaute-1,Argonaute1,hAgo1,Argonaute RISC catalytic component 1,Eukaryotic translation initiation factor 2C 1,eIF-2C 1,eIF2C 1,Putative RNA-binding protein Q99,AGO1,EIF2C1,

Tissue Specificity: Detected in blood plasma (at protein level).

Background: Eukaryotic elongation factor 2 is a protein that in humans is encoded by the EEF2 gene. This gene encodes a member of the GTP-binding translation elongation factor family. This protein is an essential factor for protein synthesis. It promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome. This protein is completely inactivated by EF-2 kinase phosporylation.

Molekulargewicht: 95 kDa

Gen-ID: 1938

UniProt: P13639

Pathways: AMPK Signaling